How to Select a Syringe Filter and How to Use it? (2020 Guide)

Posted by airekacells 17/02/2020 0 Comment(s)

How to Select a Syringe Filter and How to Use it? (2020 Guide)

  Knowledge of Syringe Filter Membrane, Pore Size, Diameter, and Usage Guide

 

 

 

In this article:

 

As a Syringe Filters Manufacturer

What is a syringe filter?

How to Select a Right Syringe Filter?

Syringe Filter Bestsellers (Stock List 2020)

  1.   PTFE Hydrophobic Syringe Filters
  2.   PTFE Hydrophilic Syringe Filters
  3.   PVDF Hydrophilic Syringe Filters
  4.   Nylon Syringe Filters
  5.   MCE Syringe Filters
  6.   PES Syringe Filters

How to Use a Syringe Filter? Instructions

Typical Particle Sizes (Chart)

 

 

 

As a Syringe Filters Manufacturer

 

Over 10 years supply of proven, and affordable syringe filters, and strong partnerships with universities and research institutes worldwide, AirekaCells offers an extensive selection of filtration products to ensure you have the reliable fundamental tools in filtering your valuable samples.

 

A wide selection of membrane materials, diameters, and both sterile and non-sterile types are available, these syringe filters offer outstanding microfiltration for applications in HPLC, water treatment, environmental, biotechnology, beverages, food, pharmaceuticals, and other fields related.  

 

All types of syringe filters listed below are ready in stock for a ONE-day ship out.

 

What is a syringe filter?

 

A syringe filter (sometimes called a wheel filter for its wheel-like shape) is a single-use membrane based filter cartridge, to be attached with a syringe to remove impurities in liquid solutions. This pre-filtration step is vital in preventing damage to instruments (e.g. liquid chromatography, ICP, etc.). 

 

Disposable syringe filters are widely used in labs for quick and efficient filtering, material purification or even sterilization for solutions <250mL, to avoid setting ups of Buchner filters or similar.

 

 

It is essential that the right selection of syringe filters is chosen to ensure reliable testing results and best purifying performance. Membrane diameters, materials, pore size, and housing all affect the right choice. Here we'll walk through the important factors to help you make the right choice compatible. 

 

 

How to Select a Right Syringe Filter?

 

Syringe Filter Quick Selection Guide

 

 

1. Determine whether your application requires pre-filtration.

 

 

2. Choose a suitable membrane type

 

Characteristics of Solution

Aqueous

Non-Polar

Protein

Nylon

Nylon

PVDF

MCE

PTFE

PES

 

PVDF

 

↑↑Click the Filter Type Name for Details of Each Membrane ↑↑

 

3. Choose Suitable Syringe Filter Diameter.

 

Volume of Samples

<10ml

<100ml

<250ml

13mm

25mm

33mm

 

4. Choose the suitable pore size.

 

Micron Size of Column

>3um

<3um

0.45um

0.22um

Check Typical Particle Sizes to Confirm Pore Sizes

Quick Navigate To Syringe Filter List Below >>

 

There are several factors to consider when choosing a syringe filter. Some of the key figures are:

 

 

1. Determine whether your application requires pre-filtration

 

2. How to select the right membrane syringe filter?

 

The following chart will help you quickly choose the proper filter membrane type.

Features of Your Applictaions:

Compatible Filter Membrane Type:

Hydrophilic Syringe Filter

PES, Nylon, MCE, Hydrophilic PTFE or PVDF

Hydrophobic Syringe Filter

PTFE, Hydrophobic PVDF

Compatible with aqueous samples only

CA, Nylon, or PES

Compatible with organic and aqueous samples

Hydrophilic PTFE, Nylon, or Hydrophilic PVDF

Compatible with gaseous samples

MCE, or PTFE

Can handle high temperature liquids <100 ℃

PES, MCE, or PTFE

Low protein binding

MCE, PES, , Hydrophilic PVDF or Hydrophilic PTFE

Nonspecific binding

Nylon or PVDF

Excellent flow rates

MCE, Nylon, PES, PTFE, or PVDF

High throughput loading

Nylon, or PTFE

Autoclave by 125℃for 15 minutes

Nylon, MCE, PES, PTFE or PVDF

 

 

3. Whats the syringe filter diameter to be chosen? 13mm or 25mm?

 

All filter inlets are female Luer-Lock compatible, have inert polypropylene housings and come in three diameters. The diameter is determined by the volume to be filtered. 

 

13 mm Syringe Filters

  • For sample volumes <10 mL range;
  • Ideal choice for most applications;
  • Holdup (dead) volume is <15 ul.
  • Female inlet luer lock
  • Maximum operation pressure <15 bar
  • Bubble point: Hydrophilic 2-3 bar; Hydrophobic 1-1.2 bar

 

25 mm Syringe Filters

  • For sample volumes <100 mL range;
  • Holdup (dead) volume is <125 ul.
  • Female inlet luer lock
  • Maximum operation pressure <10 bar
  • Bubble point: Hydrophilic 2-3 bar; Hydrophobic 1-1.2 bar

 

33 mm filters

  • For sample volumes <250 mL range;
  • Holdup (dead) volume is <180 ul.
  • Female inlet luer lock
  • 33mm filters are ideal for high throughput labs

 

 

4. Which Pore Sizes to Select? 0.22um or 0.45um?

 

The most commonly used syringe filter pore sizes are 0.2/0.22 um and 0.45 um syringe filters, for research and medical applications.

 

  1. The pore size to be used is usually determined by the particle size to be eliminated. For example, for the purpose of filtering out particulate >0.2 microns in diameter, then choose a syringe filter with a 0.2 micron pore size.
  2. Another way to determine the micron size of columns, 0.45 um for >3 um, and 0.22 um for <3um.
  3. 0.45 um membranes are typically used for general filtration and particle removal while 0.2/0.22um membranes, or sterilizing-grade membranes, are most commonly used for solution sterilization (bacteria removal).

 

Go to Typical Particle Sizes to Get Confirmed with Pore Sizes  >>

 

 

5. Sterile or Non-Sterile?

 

As we use syringe filters to eliminate unwanted particles from a solution, we can either choose sterile or non-sterile (much cheaper option), depending on the processing applications:

 

  • If a sterilized solution needed, sterile syringe filters come individually wrapped to ensure optimal performance without contamination.
  • If your solution is going to be filtered through a sterilization process, then a non-sterile syringe filter should meet your requirements.

 

 

6. Further Question?

 

Continue to check a list of our syringe filter bestsellers and please feel free to contact us for any queries: sales@airekacells.com

 

Syringe Filter Bestsellers (Stock List 2020)

 

Many different types of materials are used in syringe filters. A variety of materials, diameters, pore sizes, and in sterile and non-sterile options are on our list.

 

These syringe filters offer outstanding microfiltration for applications in HPLC, water treatment, environmental, biotechnology, beverages, food, pharmaceuticals, and other fields related.

 

The key differences are chemical compatibility, flow rate, and burst pressure (i.e. strength). The material will also give variation in the effective filtration area (EFA) and therefore the capacity of the filter.

 

The main options of syringe filters are below:

 

PTFE Hydrophobic Syringe Filters   0.22 or 0.45um

 

Hydrophobic PTFE (Polytetrafluoroethylene) syringe filters are suitable for gaseous and organic solutions, with excellent performance for high corrosive samples. It is possible for an extended sampling range using hydrophobic PTFE filters for wider chemical resistance to aggressive substances and outstanding temperature stability. A PTFE syringe filter is an ideal choice for filtering and de-gassing chromatography solvents.

 

With proper pre-wetting (commonly used alcohol), aqueous samples are also compatible. When using air-vents, these syringe filters are capable of stopping moisture from passing through. Workable pH range: 1-14.

 

  • Organic solvents with strong chemical causticity filtration
  • Strong acid solvents filtration, with an excellent flow rate
  • Alkali solvents filtration
  • Compatible with gaseous samples
  • Can handle high-temperature liquids

 

Stock List of 0.22/0.45micron PTFE Hydrophobic Syringe Filter

Type

Dia-Meter

Pore-Size

Character

Non-Sterile

Sterile

 

PTFE

13mm

0.22um

Hydrophobic

ASF12029

AFS12031S

PTFE

13mm

0.45um

Hydrophobic

ASF17028

AFS12030S

 

PTFE

25mm

0.22um

Hydrophobic

ASF12031

AFS12033S

PTFE

25mm

0.45um

Hydrophobic

ASF12030-O

AFS12032S

 

PTFE

33mm

0.22um

Hydrophobic

ASF3316TS

-

PTFE

33mm

0.45um

Hydrophobic

ASF3315TS

-

Browse All Hydrophobic PTFE Syringe Filters >>

PTFE Hydrophobic Filters Chemical Compatibility Chart >>

 

PTFE hydrophobic syringe fitler 0.22 0.45um  13 25mm

 

PTFE Hydrophilic Syringe Filters   0.2 or 0.45um

 

Resisting nearly all corrosive chemicals makes PTFE syringe filters an ideal choice. However, natural hydrophobic PTFE has it’s disadvantages not letting aqueous solutions passing through while many of these chemicals are diluted with water. The solution comes to be hydrophilic PTFE syringe filter from AirekaCells.

 

Hydrophilic PTFE syringe filters offer a choice for aqueous samples that are diluted with harsh chemicals. These filters can handle high-temperature liquids, excellent flow rates, low protein binding, and are compatible with most bases, acids and organic solvents.

 

  • Inorganic solvent with strong chemical causticity filtration
  • Mixed solvent filtration in HPLC(Acetonitrile/Water)
  • Alkali solutions filtration
  • Compatible with organic and gaseous samples
  • Low protein binding

 

Stock List of 0.22/0.45micron PTFE Hydrophilic Syringe Filter

Type

Diaemeter

Pore-Size

Color

Non-Sterile

Sterile

 

PTFE

13mm

0.22um

Hydrophilic

ASF17029

AFS12035S

PTFE

13mm

0.45um

Hydrophilic

ASF12028

AFS12034S

 

PTFE

25mm

0.22um

Hydrophilic

ASF12031-I

AFS12037S

PTFE

25mm

0.45um

Hydrophilic

ASF12030

AFS12036S

Browse All Hydrophilic PTFE Syringe Filters>>

PTFE Hydrophilic Filters Chemical Compatibility Chart >>

 

hydrophilic ptfe syringe fitler 0.22 0.45um 13/25mm

 

Chemicals Compatible with PTFE Syringe Filters

Acids

Methanol, 98%

Pyridine

Acetic, Glacial

n-Propanol

Acetonitrile

Acetic 25%

Propylene Glycol

Halogenated Hydrocarbons

Hydrochloric Concentrated

Esters

Methylene Chloride

Hydrochloric 25%

Ethyl Acetate/ Methyl Acetate

Chloroform

Sulfuric Concentrated

Amyl Propyl/ Butyle Acetate

Trichloroethylene

Sulfuric 25%

Propyl Acetate

Monochlorobenzene Freon

Nitric Concentrated

Propylene Glycol Acetate

Carbon Tetrachloride

Nitric 25%

2-Ethoxyethyl Acetate

Hydrocarbons

Phosphoric 25%

Methyl Cellosolve Acetate

Hexane Xylene

Formic 25%

Benzyl Benzoate

Toulene Benzene

Trichloroacetic 10%

Isopropyl Myristate

Kerosene Gasoline

Bases

Tricresyl Phosphate

Tetralin Decalin

Ammonium Hydroxide 25%

Organic Oxides

Ketones

Sodium Hydroxide 3 Normal

Ethyl Ether

Acetone

Alcohols

Dioxane/ Tetrahydrofuran

Cyclohexanone

Amyl Alcohol (Butanol)

Triethanolamine

Methyl Ethyl Ketone

Benzyl Alcohol

Dimethyl sulfoxide (DMSO)

Icepropylacetone

Butyl Alcohol

Isopropyl Ether

Methyl Isobutyl Ketone

Ethanol 70%

Amines and Amides

Miscellaneous

Ethanol 98%

Dimethyl Formamide

Phenol Aqueous 10%

Ethylene Glycol

Diethylacetamide

Formaldehyde Aqueous 30%

Glycerine (Glycerol)

Triethanolamine

Hydrogen Peroxide 30%

Isopropanol

Aniline

Silicone Oil & Mineral Oil

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Browse All PTFE Syringe Filters >>

 

PVDF Hydrophilic Syringe Filters  0.22um or 0.45um

 

Hydrophilic PVDF (Polyvinylidene Fluoride) is an optimal choice for protein-based samples with high non-specific binding, HPLC (High Performance Liquid Chromatography) and UHPLC (Ultra HPLC).

 

Our PVDF filters are high defined pore structure, low nonspecific binding, compatible with organic and aqueous solvents. Please note that Hydrophilic PVDF syringe filters are not compatible with majority of strong acids and caustic solutions, such as dimethyl sulfoxide, dimethylformamide (DMF), acetone, ketones, esters, and ethers.

 

  • Compatible with organic and aqueous samples
  • Compatible with gaseous samples
  • Low protein binding
  • Nonspecific binding
  • Excellent flow rates
  • Well-defined pore structure
  • High-temperature filtration

 

Please check the compatible chemicals as chart below.

 

Stock List of 0.22/ 0.45micron PVDF Hydrophilic Syringe Filter

Type

Dia-meter

Pore-Size

Color

Non-Sterile

Sterile

 

PVDF

13mm

0.22um

Hydrophilic

ASF12037

AFS12027S

PVDF

13mm

0.45um

Hydrophilic

ASF12036

AFS12026S

 

PVDF

25mm

0.22um

Hydrophilic

ASF12039

AFS12029S

PVDF

25mm

0.45um

Hydrophilic

ASF12038

AFS12028S

 

PVDF

33mm

0.22um

Hydrophilic

ASF3324

-

PVDF

33mm

0.45um

Hydrophilic

ASF3323

-

Hydrophobic PVDF are also available on request, please email: sales@airekacells.com

Browse All Hydrophilic PVDF Syringe Filters >>

PVDF Hydrophilic Filters Chemical Compatibility Chart >>

 

PVDF Syringe Filters 0.22 0.45um 

Chemicals Compatible with PVDF Syringe Filters

Acids

Ethanol, 98%

Halogenated Hydrocarbons

Acetic, Glacial

Ethylene Glycol

Methylene Chloride

Acetic, 25%

Glycerine (Glycerol)

Chloroform

Hydro-chloric, Concentrated

Isopropanol

Trichloroethylene

Hydro-chloric, 25%

Methanol, 98%

Mono-chloro-benzene, Freon

Sulfuric, 25%

n-Propanol

Carbon Tetra-chloride

Nitric, Concentrated

Propylene Glycol

Hydro-carbons

Nitric, 25%

Esters

Hexane, Xylene

Bases

Ethyl Acetate/ Methyl Acetate

Toulene, Benzene

Sodium Hydroxide, 3 Normal

Organic Oxides

Kerosene, Gasoline

Alcohols

Ethyl Ether

Tetralin, Decalin

Amyl Alcohol (Butanol)

Isopropyl Ether

Ketones

Benzyl Alcohol

Amines and Amides

Miscellaneous

Butyl Alcohol

Aceto-nitrile

Formaldehyde Aqueous, 30%

Ethanol, 70%

 

Silicone Oil & Mineral Oil

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Browse All Hydrophilic PVDF Syringe Filters >>

 

Nylon Syringe Filters >>  0.22um-0.45um-0.8um

 

Nylon syringe filer is naturally hydrophilic. For most aqueous and organic slovens in the lab, the Nylon syringe filters are compatible. Excellent flow rate and high throughput loading and affordable cost make nylon syringe filters a common choice for semiconductor industrial applications.

 

This type of nylon filter is not optimal for protein samples with high non-specific binding. Nylon can be sterilized by autoclaving at 120°C, gamma radiation, or ethylene oxide. Please check the compatible chemicals below in the chart.

 

  • Compatible with aqueous samples only
  • Nonspecific binding
  • Excellent flow rates
  • High throughput loading
  • Electric semiconductor industrial water filtration
  • Chemicals filtration
  • Beverage filtration

 

Stock List of 0.22/0.45micron Nylon Hydrophilic Syringe Filter

Type

Dia-meter

Pore-Size

Character

Non-Sterile

Sterile

 

Nylon

13mm

0.22um

Hydrophilic

ASF12011

AFS12015S

Nylon

13mm

0.45um

Hydrophilic

ASF12010

AFS12014S

Nylon

13mm

0.8um

Hydrophilic

ASF12046

-

 

Nylon

25mm

0.22um

Hydrophilic

ASF12015

AFS12017S

Nylon

25mm

0.45um

Hydrophilic

ASF12014

AFS12016S

Nylon

25mm

0.8um

Hydrophilic

ASF12047

-

 

Nylon

33mm

0.22um

Hydrophilic

ASF3302

-

Nylon

33mm

0.45um

Hydrophilic

ASF3301

-

Browse All Nylon Syringe Filters>>

Nylon Syringe Filters Chemical Compatibility Chart >>

 

Nylon Syringe Filter 0.22 0.45 micron

 

Chemicals Compatible with Nylon Syringe Filters

Acids

Ethyl Acetate/Methyl Acetate

Trichloroethylene

Acetic, 25%

Amyl Propyl/Butyle Acetate

Monochlorobenzene, Freon

Bases

Propyl Acetate

Carbon Tetrachloride

Ammonium Hydroxide, 25%

Benzyl Benzoate

Hydrocarbons

Sodium Hydroxide, 3 Normal

Isopropyl Myristate

Hexane, Xylene

Alcohols

Organic Oxides

Toulene, Benzene

Amyl Alcohol (Butanol)

Ethyl Ether

Kerosene, Gasoline

Benzyl Alcohol

Dioxane/Tetrahydrofuran

Ketones

Butyl Alcohol

Triethanolamine

Acetone

Ethanol, 98%

Dimethylsulfoxide (DMSO)

Cyclohexanone

Ethylene Glycol

Amines and Amides

Methyl Ethyl Ketone

Glycerine (Glycerol)

Diethylacetamide

Icepropylacetone

Isopropanol

Triethanolamine

Miscellaneous

Methanol, 98%

Pyridine

Formaldehyde Aqueous, 30%

n-Propanol

Acetonitrile

Hydrogen Peroxide, 30%

Propylene Glycol

Halogenated Hydrocarbons

 

Esters

Chloroform

 

Browse All Nylon Syringe Filters>>

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MCE Syringe Filters

 

MCE (Mixed Cellulose Esters) Syringe Filter is very low protein binding which makes it ideal for aqueous based samples. MCE is an outstanding choice for maximum protein recovery. Lab studies show that MCE bind even less protein than PVDF or PES syringe filters.

 

MCE syringe filters also have excellent flow rates but with limited organic solvent or benzyl alcohol resistance. Workable pH range ~4-8.

 

  • Filtration of tissue culture media and sensitive biological samples
  • Gas particulate and bacteria filtration
  • Oil particulate and bacteria filtration
  • Alcohol particulate and bacteria filtration
  • Other solvent particulate and bacteria filtration

 

Stock List of 0.22/0.45micron MCE Hydrophilic Syringe Filter

Type

Diaemeter

Pore-Size

Character

Non-Sterile

Sterile

 

MCE

13mm

0.22um

Hydrophilic

ASF12021

AFS12019S

MCE

13mm

0.45um

Hydrophilic

ASF12020

AFS12018S

 

MCE

25mm

0.22um

Hydrophilic

ASF12023

AFS12021S

MCE

25mm

0.45um

Hydrophilic

ASF12022

AFS12020S

 

MCE

33mm

0.22um

Hydrophilic

ASF3314

-

MCE

33mm

0.45um

Hydrophilic

ASF3313

-

Browse All MCE Syringe Filters>>

MCE Syringe Filters Chemical Compatibility Chart >>

 

MCE syringe filters 0.22 0.45um 13/25mm

 

Chemicals Compatible with MCE Syringe Filters

Acids

Methanol, 98%

Trichloroethylene

Phosphoric, 25%

n-Propanol

Monochlorobenzene, Freon

Trichloroacetic, 10%

Esters

Hydrocarbons

Bases

Benzyl Benzoate

Hexane, Xylene

Ammonium Hydroxide, 25%

Isopropyl Myristate

Toulene, Benzene

Alcohols

Tricresyl Phosphate

Kerosene, Gasoline

Amyl Alcohol (Butanol)

Organic Oxides

Tetralin, Decalin

Butyl Alcohol

Ethyl Ether

Ketones

Ethanol, 70%

Triethanolamine

Icepropylacetone

Ethanol, 98%

Isopropyl Ether

Miscellaneous

Ethylene Glycol

Amines and Amides

Formaldehyde Aqueous, 30%

Glycerine (Glycerol)

Triethanolamine

Hydrogen Peroxide, 30%

Isopropanol

Halogenated Hydrocarbons

Silicone Oil & Mineral Oil

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Browse All MCE Syringe Filters>>

 

PES Syringe Filters

 

PES (Polyethersulfone ) syringe filter is natural hydrophilic which makes is an ideal choice (highly recommended) for aqueous solutions and alcohols. It’s not necessary to treat the filter membrane surface with wetting agents.

 

With PES syringe filter membrane mechanically strong character, it can handle high-temperature liquids, with great flow rate (better than PTFE), and low protein binding. With these features, PES syringe filters offer superior performance for use with biological samples and media culture. Workable pH range ~3-14.

 

  • Compatible with organic and aqueous samples
  • Mechanically strong for high flow rate
  • Low protein binding
  • Tissue culture media filtration
  • High-temperature filtration
  • Sterile filtering protein solution
  • Tissue culture additive filtration

 

Check PES Syringe Filter Chemical Compatibility Chart Below.

 

Stock List of 0.22/0.45micron PES Hydrophilic Syringe Filter

Type

Dia-meter

Pore-Size

Character

Non-Sterile

Sterile

 

PES

13mm

0.22um

Hydrophilic

ASF12013

AFS12011S

PES

13mm

0.45um

Hydrophilic

ASF12012

AFS12010S

PES

13mm

0.8um

Hydrophilic

ASF12044

-

 

PES

25mm

0.22um

Hydrophilic

ASF12017

AFS12013S

PES

25mm

0.45um

Hydrophilic

ASF12016

AFS12012S

PES

25mm

0.8um

Hydrophilic

ASF12045

-

 

PES

33mm

0.22um

Hydrophilic

ASF3304

-

PES

33mm

0.45um

Hydrophilic

ASF3303

-

Browse All PES Syringe Filters>>

PES Syringe Filters Chemical Compatibility Chart >>

 

PES syringe filters

 

Chemicals Compatible with PES Syringe Filters

Acids

Amyl Alcohol (Butanol)

Organic Oxides

Acetic, Glacial

Butyl Alcohol

Ethyl Ether

Acetic, 25%

Ethanol, 70%

Isopropyl Ether

Hydrochloric, Concentrated

Ethanol, 98%

Amines and Amides

Hydrochloric, 25%

Ethylene Glycol

Halogenated Hydrocarbons

Sulfuric, 25%

Glycerine (Glycerol)

Hydrocarbons

Nitric, 25%

Isopropanol

Ketones

Bases

Methanol, 98%

Miscellaneous

Ammonium Hydroxide, 25%

n-Propanol

Formaldehyde Aqueous, 30%

Sodium Hydroxide, 3 Normal

Propylene Glycol

Silicone Oil & Mineral Oil

Alcohols

Esters

 

Please Switch to Desktop Version if You Cannot View the Form Correctly.

Browse All PES Syringe Filters>>

 

How to Use a Syringe Filter? Instructions

 

As syringe filters are very commonly used in the lab, sometimes improper use of these filters might cause unnecessary trouble, such as sample leakage, filter breakage, or filter falling off the syringe. This instruction aims to provide a step by step guides for researchers new to syringe filter.

 

Step 1: Open Syringe

  • Open the syringe package and remove the syringe needle. A syringe with a luer-lock tip is recommended. Syringe Volume depends on the samples to be filtered.

Step 2: Attach a syringe filter to the Syringe

  • Open the syringe filter package so that you can later pick the filter up easily, especially for individually packed sterile syringe filters.

a. For sample volume < 10 mL

  1. Draw a small amount of air (about 1 mL) into the syringe before loading the sample solution. The air is used to purge the filter to minimize hold up volume.
  2. Attach the filter securely to the syringe using a clockwise motion.

Note: Please use caution when using small volume syringes as they can generate high pressure.

 

b. For sample volume≥ 10 mL

  1. Remove the plunger from the syringe.
  2. Attach the filter securely to the syringe using a twisting motion.
  3. Pour the sample solution from the unpreserved fill bottle into the top of the syringe leaving space for the plunger. 
  4. Reinsert the plunger.

Note: The individually packed sterile syringe filter can be held in the original package to minimize contamination while attaching the syringe.

 

Step 3: Secure the Syringe Filter

  • Secure the syringe filter using a clockwise motion with luer-lock syringe. DO NOT overtighten.

Step 4: Filter the Solution

  • Hold the assembled syringe and filter it upright. Filter the solution into the receiving bottle by slowly pressing down the syringe plunger to push the sample through the filter.

Note: Avoid pressing excessively as this could cause the filter housing to burst.

Step 5: Filter more Samples

  1. If more solutions need to be filtered, detach the syringe filter from the syringe tip.
  2. Reattach the syringe filter with a clockwise motion.
  3. Pour the sample solution from the top of the syringe leaving space for the plunger.
  4. Reinsert the plunger and continue filtering.

Note: If the backpressure increases significantly, change the filter as it may have been clogged.

Step 6: Discard the Filter

  • Detach and discard the used syringe filter.

 

Typical Particle Sizes

 

Typical Particle Sizes

 

Gelatinous Precipitates

µm

Metal hydroxides

25–40

Precipitated silica

25–40

Crystalline Precipitates

 

Ammonium phosphomolybdate

20

Calcium oxatate

15

Lead sulphate

10

Barium sulphate (hot ppt.)

8

Barium sulphate (cold ppt.)

3

Blood Cells

 

Platelets

2–3

Erythrocytes (average)

7.0

Polymorphs

8–12

Small lymphocytes

7–10

Large lymphocytes

12–15

Monocytes

16–22

Bacteria*

 

Cocci

0.5

Bacilli

1.0 x (1.0–1.0)

Serratia marcescens

0.5 x (0.5–1.0)

Pneumococcus

1.0

Bacillius tuberculosis

0.3 x (2.5–3.5)

Amoeba

12-30

E.coli

0.5 x (1.0–3.0)

Smallest bacteria

0.22

Other Microorganisms, etc.

 

Yeast cells

2.0–8.0

Tobacco smoke

0.5

Colloids

0.06–0.30

Rye grass pollen

34

Ragweed pollen

20

Puffball spores

3.3

GO BACK to Syringe Filter Product List >>

 

 


This document is believed to be accurate and up-to-date. However, Agilent Technologies, Inc. cannot assume responsibility for the use of this material.

The information contained herein is intended for use by informed individuals who can and must determine its fitness for their purpose.