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What are “short path” cuvettes and why are they used? (sample too concentrated)
In the case of high-concentration samples, diluting the sample allows measurement with a 10-mm cell. There are samples, however, that cannot be diluted easily. For example, due to interaction with the solvent, diluting a sample may cause a change in the absorbance (i.e., a shift in the peak wavelengths).
In cases where the absorbance is high, and dilution is difficult, measurement using a "short-path cell" is effective. There are short-path cells with optical path lengths of 1 mm, 2 mm, and 5 mm. The figure shows the results obtained by analyzing toluene with a 1-mm cell and a 10-mm cell. It can be seen that there is much less absorption saturation (i.e., 0 % transmittance) with the 1-mm cell than there is with the 10-mm cell.
A well-known application of measurement using a short-path cell is solution analysis in the near-infrared region. If a 10-mm cell is used for measurement in the near-infrared region, saturation often occurs due to absorption by the solvent, making it impossible to ascertain the absorption of the sample. A short-path cell is used to prevent absorption saturation due to the solvent.
With low- and high-concentration samples, the optical path length of the cell is selected in accordance with the size of the absorbance or transmittance.
Cuvette, 2 windows, Volume: 1.7ml, Outer Dim(HxWxD): 45x12.5x7.5mm, Path Length: 5mm, Width: 10mm, ES Quartz Glass: 190nm-2500nm ..
US$59.00 US$32.00
Cuvette, 4 windows, Volume: 1.7ml, Outer Dim(HxWxD): 45x12.5x7.5mm, Path Length: 5mm, Width: 10mm, ES Quartz Glass: 190nm-2500nm..
US$68.00 US$38.00
Cuvette, 4 windows, Volume: 1.7ml, Outer Dim(HxWxD): 45x12.5x7.5mm, Path Length: 5mm, Width: 10mm, with teflon stopper, ES Quartz Glass: 190nm-2500nm..
US$161.20 US$129.00
Cuvette, 2 windows, Volume: 1.7ml, teflon screw cap, PTFE septa, Outer Dim(HxWxD): 45x12.5x12.5mm, Path Length: 5mm, Width: 10mm, ES Quartz Glass: 190nm-2500nm..
US$234.00 US$99.00
Cuvette, 2 windows, Volume: 1.7ml, screw cap with 2mm center hole, PTFE septa, Outer Dim(HxWxD): 45x12.5x7.5mm, Path Length: 5mm, Width: 10mm, ES Quartz Glass: 190nm-2500nm..
US$234.00 US$119.00
Cuvette, 2 windows, Volume: 350ul, Outer Dim(HxWxD): 45x12.5x3.5mm, Path Length: 1mm, Width: 10mm, ES Quartz Glass: 190nm-2500nm..
US$75.00 US$48.00
Cuvette, 2 windows, Volume: 0.35ml, Outer Dim(HxWxD): 45x12.5x3.5mm, Path Length: 1mm, Width: 10mm, with teflon stopper, ES Quartz Glass: 190nm-2500nm..
US$245.00 US$148.00
Cuvette, 2 windows, Volume: 0.35ml, Outer Dim(HxWxD): 45x12.5x3.5mm, Path Length: 1mm, Width: 10mm, with teflon screw cap, ES Quartz Glass: 200nm-2500nm..
US$292.00 US$158.00
Cuvette, 2 windows, Volume: 700ul, Outer Dim(HxWxD): 45x12.5x4.5mm, Path Length: 2mm, Width: 10mm, ES Quartz Glass: 190nm-2500nm..
US$245.00 US$138.00