Your shopping cart is empty!
Knowledge of Syringe Filter Membrane, Pore Size, Diameter, and Usage Guide
Over 10 years supply of proven, and affordable syringe filters, and strong partnerships with universities and research institutes worldwide, AirekaCells offers an extensive selection of filtration products to ensure you have the reliable fundamental tools in filtering your valuable samples.
A wide selection of membrane materials, diameters, and both sterile and non-sterile types are available, these syringe filters offer outstanding microfiltration for applications in HPLC, water treatment, environmental, biotechnology, beverages, food, pharmaceuticals, and other fields related.
All types of syringe filters listed below are ready in stock for a ONE-day ship out.
A syringe filter (sometimes called a wheel filter for its wheel-like shape) is a single-use membrane based filter cartridge, to be attached with a syringe to remove impurities in liquid solutions. This pre-filtration step is vital in preventing damage to instruments (e.g. liquid chromatography, ICP, etc.).
Disposable syringe filters are widely used in labs for quick and efficient filtering, material purification or even sterilization for solutions <250mL, to avoid setting ups of Buchner filters or similar.
It is essential that the right selection of syringe filters is chosen to ensure reliable testing results and best purifying performance. Membrane diameters, materials, pore size, and housing all affect the right choice. Here we'll walk through the important factors to help you make the right choice compatible.
Syringe Filter Quick Selection Guide
1. Determine whether your application requires pre-filtration.
| |||
2. Choose a suitable membrane type
| |||
Characteristics of Solution | |||
Aqueous | Non-Polar | Protein | |
|
| ||
↑↑Click the Filter Type Name for Details of Each Membrane ↑↑ | |||
3. Choose Suitable Syringe Filter Diameter.
| |||
Volume of Samples | |||
<10ml | <100ml | <250ml | |
13mm | 25mm | 33mm | |
4. Choose the suitable pore size.
| |||
Micron Size of Column | |||
>3um | <3um | ||
0.45um | 0.22um | ||
Check Typical Particle Sizes to Confirm Pore Sizes |
Quick Navigate To Syringe Filter List Below >>
There are several factors to consider when choosing a syringe filter. Some of the key figures are:
The following chart will help you quickly choose the proper filter membrane type.
Features of Your Applictaions: | Compatible Filter Membrane Type: |
---|---|
Hydrophilic Syringe Filter | PES, Nylon, MCE, Hydrophilic PTFE or PVDF |
Hydrophobic Syringe Filter | PTFE, Hydrophobic PVDF |
Compatible with aqueous samples only | CA, Nylon, or PES |
Compatible with organic and aqueous samples | Hydrophilic PTFE, Nylon, or Hydrophilic PVDF |
Compatible with gaseous samples | MCE, or PTFE |
Can handle high temperature liquids <100 ℃ | PES, MCE, or PTFE |
Low protein binding | MCE, PES, , Hydrophilic PVDF or Hydrophilic PTFE |
Nonspecific binding | Nylon or PVDF |
Excellent flow rates | MCE, Nylon, PES, PTFE, or PVDF |
High throughput loading | Nylon, or PTFE |
Autoclave by 125℃for 15 minutes | Nylon, MCE, PES, PTFE or PVDF |
All filter inlets are female Luer-Lock compatible, have inert polypropylene housings and come in three diameters. The diameter is determined by the volume to be filtered.
The most commonly used syringe filter pore sizes are 0.2/0.22 um and 0.45 um syringe filters, for research and medical applications.
Go to Typical Particle Sizes to Get Confirmed with Pore Sizes >>
As we use syringe filters to eliminate unwanted particles from a solution, we can either choose sterile or non-sterile (much cheaper option), depending on the processing applications:
Continue to check a list of our syringe filter bestsellers and please feel free to contact us for any queries: sales@airekacells.com
Many different types of materials are used in syringe filters. A variety of materials, diameters, pore sizes, and in sterile and non-sterile options are on our list.
These syringe filters offer outstanding microfiltration for applications in HPLC, water treatment, environmental, biotechnology, beverages, food, pharmaceuticals, and other fields related.
The key differences are chemical compatibility, flow rate, and burst pressure (i.e. strength). The material will also give variation in the effective filtration area (EFA) and therefore the capacity of the filter.
The main options of syringe filters are below:
Hydrophobic PTFE (Polytetrafluoroethylene) syringe filters are suitable for gaseous and organic solutions, with excellent performance for high corrosive samples. It is possible for an extended sampling range using hydrophobic PTFE filters for wider chemical resistance to aggressive substances and outstanding temperature stability. A PTFE syringe filter is an ideal choice for filtering and de-gassing chromatography solvents.
With proper pre-wetting (commonly used alcohol), aqueous samples are also compatible. When using air-vents, these syringe filters are capable of stopping moisture from passing through. Workable pH range: 1-14.
Type | Dia-Meter | Pore-Size | Character | Non-Sterile | Sterile |
| |||||
PTFE | 13mm | 0.22um | Hydrophobic | ||
PTFE | 13mm | 0.45um | Hydrophobic | ||
| |||||
PTFE | 25mm | 0.22um | Hydrophobic | ||
PTFE | 25mm | 0.45um | Hydrophobic | ||
| |||||
PTFE | 33mm | 0.22um | Hydrophobic | - | |
PTFE | 33mm | 0.45um | Hydrophobic | - |
Browse All Hydrophobic PTFE Syringe Filters >>
PTFE Hydrophobic Filters Chemical Compatibility Chart >>
Resisting nearly all corrosive chemicals makes PTFE syringe filters an ideal choice. However, natural hydrophobic PTFE has it’s disadvantages not letting aqueous solutions passing through while many of these chemicals are diluted with water. The solution comes to be hydrophilic PTFE syringe filter from AirekaCells.
Hydrophilic PTFE syringe filters offer a choice for aqueous samples that are diluted with harsh chemicals. These filters can handle high-temperature liquids, excellent flow rates, low protein binding, and are compatible with most bases, acids and organic solvents.
Type | Diaemeter | Pore-Size | Color | Non-Sterile | Sterile |
| |||||
PTFE | 13mm | 0.22um | Hydrophilic | ||
PTFE | 13mm | 0.45um | Hydrophilic | ||
| |||||
PTFE | 25mm | 0.22um | Hydrophilic | ||
PTFE | 25mm | 0.45um | Hydrophilic |
Browse All Hydrophilic PTFE Syringe Filters>>
PTFE Hydrophilic Filters Chemical Compatibility Chart >>
Chemicals Compatible with PTFE Syringe Filters
Acids | Methanol, 98% | Pyridine |
Acetic, Glacial | n-Propanol | Acetonitrile |
Acetic 25% | Propylene Glycol | Halogenated Hydrocarbons |
Hydrochloric Concentrated | Esters | Methylene Chloride |
Hydrochloric 25% | Ethyl Acetate/ Methyl Acetate | Chloroform |
Sulfuric Concentrated | Amyl Propyl/ Butyle Acetate | Trichloroethylene |
Sulfuric 25% | Propyl Acetate | Monochlorobenzene Freon |
Nitric Concentrated | Propylene Glycol Acetate | Carbon Tetrachloride |
Nitric 25% | 2-Ethoxyethyl Acetate | Hydrocarbons |
Phosphoric 25% | Methyl Cellosolve Acetate | Hexane Xylene |
Formic 25% | Benzyl Benzoate | Toulene Benzene |
Trichloroacetic 10% | Isopropyl Myristate | Kerosene Gasoline |
Bases | Tricresyl Phosphate | Tetralin Decalin |
Ammonium Hydroxide 25% | Organic Oxides | Ketones |
Sodium Hydroxide 3 Normal | Ethyl Ether | Acetone |
Alcohols | Dioxane/ Tetrahydrofuran | Cyclohexanone |
Amyl Alcohol (Butanol) | Triethanolamine | Methyl Ethyl Ketone |
Benzyl Alcohol | Dimethyl sulfoxide (DMSO) | Icepropylacetone |
Butyl Alcohol | Isopropyl Ether | Methyl Isobutyl Ketone |
Ethanol 70% | Amines and Amides | Miscellaneous |
Ethanol 98% | Dimethyl Formamide | Phenol Aqueous 10% |
Ethylene Glycol | Diethylacetamide | Formaldehyde Aqueous 30% |
Glycerine (Glycerol) | Triethanolamine | Hydrogen Peroxide 30% |
Isopropanol | Aniline | Silicone Oil & Mineral Oil |
Please Switch to Desktop Version if You Cannot View the Form Correctly.
Browse All PTFE Syringe Filters >>
Hydrophilic PVDF (Polyvinylidene Fluoride) is an optimal choice for protein-based samples with high non-specific binding, HPLC (High Performance Liquid Chromatography) and UHPLC (Ultra HPLC).
Our PVDF filters are high defined pore structure, low nonspecific binding, compatible with organic and aqueous solvents. Please note that Hydrophilic PVDF syringe filters are not compatible with majority of strong acids and caustic solutions, such as dimethyl sulfoxide, dimethylformamide (DMF), acetone, ketones, esters, and ethers.
Please check the compatible chemicals as chart below.
Type | Dia-meter | Pore-Size | Color | Non-Sterile | Sterile |
| |||||
PVDF | 13mm | 0.22um | Hydrophilic | ||
PVDF | 13mm | 0.45um | Hydrophilic | ||
| |||||
PVDF | 25mm | 0.22um | Hydrophilic | ||
PVDF | 25mm | 0.45um | Hydrophilic | ||
| |||||
PVDF | 33mm | 0.22um | Hydrophilic | - | |
PVDF | 33mm | 0.45um | Hydrophilic | - |
Hydrophobic PVDF are also available on request, please email: sales@airekacells.com
Browse All Hydrophilic PVDF Syringe Filters >>
PVDF Hydrophilic Filters Chemical Compatibility Chart >>
Chemicals Compatible with PVDF Syringe Filters
Acids | Ethanol, 98% | Halogenated Hydrocarbons |
Acetic, Glacial | Ethylene Glycol | Methylene Chloride |
Acetic, 25% | Glycerine (Glycerol) | Chloroform |
Hydro-chloric, Concentrated | Isopropanol | Trichloroethylene |
Hydro-chloric, 25% | Methanol, 98% | Mono-chloro-benzene, Freon |
Sulfuric, 25% | n-Propanol | Carbon Tetra-chloride |
Nitric, Concentrated | Propylene Glycol | Hydro-carbons |
Nitric, 25% | Esters | Hexane, Xylene |
Bases | Ethyl Acetate/ Methyl Acetate | Toulene, Benzene |
Sodium Hydroxide, 3 Normal | Organic Oxides | Kerosene, Gasoline |
Alcohols | Ethyl Ether | Tetralin, Decalin |
Amyl Alcohol (Butanol) | Isopropyl Ether | Ketones |
Benzyl Alcohol | Amines and Amides | Miscellaneous |
Butyl Alcohol | Aceto-nitrile | Formaldehyde Aqueous, 30% |
Ethanol, 70% |
| Silicone Oil & Mineral Oil |
Please Switch to Desktop Version if You Cannot View the Form Correctly.
Browse All Hydrophilic PVDF Syringe Filters >>
Nylon syringe filer is naturally hydrophilic. For most aqueous and organic slovens in the lab, the Nylon syringe filters are compatible. Excellent flow rate and high throughput loading and affordable cost make nylon syringe filters a common choice for semiconductor industrial applications.
This type of nylon filter is not optimal for protein samples with high non-specific binding. Nylon can be sterilized by autoclaving at 120°C, gamma radiation, or ethylene oxide. Please check the compatible chemicals below in the chart.
Type | Dia-meter | Pore-Size | Character | Non-Sterile | Sterile |
| |||||
Nylon | 13mm | 0.22um | Hydrophilic | ||
Nylon | 13mm | 0.45um | Hydrophilic | ||
Nylon | 13mm | 0.8um | Hydrophilic | - | |
| |||||
Nylon | 25mm | 0.22um | Hydrophilic | ||
Nylon | 25mm | 0.45um | Hydrophilic | ||
Nylon | 25mm | 0.8um | Hydrophilic | - | |
| |||||
Nylon | 33mm | 0.22um | Hydrophilic | - | |
Nylon | 33mm | 0.45um | Hydrophilic | - |
Browse All Nylon Syringe Filters>>
Nylon Syringe Filters Chemical Compatibility Chart >>
Chemicals Compatible with Nylon Syringe Filters
Acids | Ethyl Acetate/Methyl Acetate | Trichloroethylene |
Acetic, 25% | Amyl Propyl/Butyle Acetate | Monochlorobenzene, Freon |
Bases | Propyl Acetate | Carbon Tetrachloride |
Ammonium Hydroxide, 25% | Benzyl Benzoate | Hydrocarbons |
Sodium Hydroxide, 3 Normal | Isopropyl Myristate | Hexane, Xylene |
Alcohols | Organic Oxides | Toulene, Benzene |
Amyl Alcohol (Butanol) | Ethyl Ether | Kerosene, Gasoline |
Benzyl Alcohol | Dioxane/Tetrahydrofuran | Ketones |
Butyl Alcohol | Triethanolamine | Acetone |
Ethanol, 98% | Dimethylsulfoxide (DMSO) | Cyclohexanone |
Ethylene Glycol | Amines and Amides | Methyl Ethyl Ketone |
Glycerine (Glycerol) | Diethylacetamide | Icepropylacetone |
Isopropanol | Triethanolamine | Miscellaneous |
Methanol, 98% | Pyridine | Formaldehyde Aqueous, 30% |
n-Propanol | Acetonitrile | Hydrogen Peroxide, 30% |
Propylene Glycol | Halogenated Hydrocarbons |
|
Esters | Chloroform |
|
Browse All Nylon Syringe Filters>>
Please Switch to Desktop Version if You Cannot View the Form Correctly.
MCE (Mixed Cellulose Esters) Syringe Filter is very low protein binding which makes it ideal for aqueous based samples. MCE is an outstanding choice for maximum protein recovery. Lab studies show that MCE bind even less protein than PVDF or PES syringe filters.
MCE syringe filters also have excellent flow rates but with limited organic solvent or benzyl alcohol resistance. Workable pH range ~4-8.
Type | Diaemeter | Pore-Size | Character | Non-Sterile | Sterile |
| |||||
MCE | 13mm | 0.22um | Hydrophilic | ||
MCE | 13mm | 0.45um | Hydrophilic | ||
| |||||
MCE | 25mm | 0.22um | Hydrophilic | ||
MCE | 25mm | 0.45um | Hydrophilic | ||
| |||||
MCE | 33mm | 0.22um | Hydrophilic | - | |
MCE | 33mm | 0.45um | Hydrophilic | - |
Browse All MCE Syringe Filters>>
MCE Syringe Filters Chemical Compatibility Chart >>
Chemicals Compatible with MCE Syringe Filters
Acids | Methanol, 98% | Trichloroethylene |
Phosphoric, 25% | n-Propanol | Monochlorobenzene, Freon |
Trichloroacetic, 10% | Esters | Hydrocarbons |
Bases | Benzyl Benzoate | Hexane, Xylene |
Ammonium Hydroxide, 25% | Isopropyl Myristate | Toulene, Benzene |
Alcohols | Tricresyl Phosphate | Kerosene, Gasoline |
Amyl Alcohol (Butanol) | Organic Oxides | Tetralin, Decalin |
Butyl Alcohol | Ethyl Ether | Ketones |
Ethanol, 70% | Triethanolamine | Icepropylacetone |
Ethanol, 98% | Isopropyl Ether | Miscellaneous |
Ethylene Glycol | Amines and Amides | Formaldehyde Aqueous, 30% |
Glycerine (Glycerol) | Triethanolamine | Hydrogen Peroxide, 30% |
Isopropanol | Halogenated Hydrocarbons | Silicone Oil & Mineral Oil |
Please Switch to Desktop Version if You Cannot View the Form Correctly.
Browse All MCE Syringe Filters>>
PES (Polyethersulfone ) syringe filter is natural hydrophilic which makes is an ideal choice (highly recommended) for aqueous solutions and alcohols. It’s not necessary to treat the filter membrane surface with wetting agents.
With PES syringe filter membrane mechanically strong character, it can handle high-temperature liquids, with great flow rate (better than PTFE), and low protein binding. With these features, PES syringe filters offer superior performance for use with biological samples and media culture. Workable pH range ~3-14.
Check PES Syringe Filter Chemical Compatibility Chart Below.
Type | Dia-meter | Pore-Size | Character | Non-Sterile | Sterile |
| |||||
PES | 13mm | 0.22um | Hydrophilic | ||
PES | 13mm | 0.45um | Hydrophilic | ||
PES | 13mm | 0.8um | Hydrophilic | - | |
| |||||
PES | 25mm | 0.22um | Hydrophilic | ||
PES | 25mm | 0.45um | Hydrophilic | ||
PES | 25mm | 0.8um | Hydrophilic | - | |
| |||||
PES | 33mm | 0.22um | Hydrophilic | - | |
PES | 33mm | 0.45um | Hydrophilic | - |
Browse All PES Syringe Filters>>
PES Syringe Filters Chemical Compatibility Chart >>
Chemicals Compatible with PES Syringe Filters
Acids | Amyl Alcohol (Butanol) | Organic Oxides |
Acetic, Glacial | Butyl Alcohol | Ethyl Ether |
Acetic, 25% | Ethanol, 70% | Isopropyl Ether |
Hydrochloric, Concentrated | Ethanol, 98% | Amines and Amides |
Hydrochloric, 25% | Ethylene Glycol | Halogenated Hydrocarbons |
Sulfuric, 25% | Glycerine (Glycerol) | Hydrocarbons |
Nitric, 25% | Isopropanol | Ketones |
Bases | Methanol, 98% | Miscellaneous |
Ammonium Hydroxide, 25% | n-Propanol | Formaldehyde Aqueous, 30% |
Sodium Hydroxide, 3 Normal | Propylene Glycol | Silicone Oil & Mineral Oil |
Alcohols | Esters |
|
Please Switch to Desktop Version if You Cannot View the Form Correctly.
Browse All PES Syringe Filters>>
As syringe filters are very commonly used in the lab, sometimes improper use of these filters might cause unnecessary trouble, such as sample leakage, filter breakage, or filter falling off the syringe. This instruction aims to provide a step by step guides for researchers new to syringe filter.
Note: Please use caution when using small volume syringes as they can generate high pressure.
Note: The individually packed sterile syringe filter can be held in the original package to minimize contamination while attaching the syringe.
Note: Avoid pressing excessively as this could cause the filter housing to burst.
Note: If the backpressure increases significantly, change the filter as it may have been clogged.
Typical Particle Sizes |
|
Gelatinous Precipitates | µm |
Metal hydroxides | 25–40 |
Precipitated silica | 25–40 |
Crystalline Precipitates |
|
Ammonium phosphomolybdate | 20 |
Calcium oxatate | 15 |
Lead sulphate | 10 |
Barium sulphate (hot ppt.) | 8 |
Barium sulphate (cold ppt.) | 3 |
Blood Cells |
|
Platelets | 2–3 |
Erythrocytes (average) | 7.0 |
Polymorphs | 8–12 |
Small lymphocytes | 7–10 |
Large lymphocytes | 12–15 |
Monocytes | 16–22 |
Bacteria* |
|
Cocci | 0.5 |
Bacilli | 1.0 x (1.0–1.0) |
Serratia marcescens | 0.5 x (0.5–1.0) |
Pneumococcus | 1.0 |
Bacillius tuberculosis | 0.3 x (2.5–3.5) |
Amoeba | 12-30 |
E.coli | 0.5 x (1.0–3.0) |
Smallest bacteria | 0.22 |
Other Microorganisms, etc. |
|
Yeast cells | 2.0–8.0 |
Tobacco smoke | 0.5 |
Colloids | 0.06–0.30 |
Rye grass pollen | 34 |
Ragweed pollen | 20 |
Puffball spores | 3.3 |
GO BACK to Syringe Filter Product List >>
This document is believed to be accurate and up-to-date. However, Agilent Technologies, Inc. cannot assume responsibility for the use of this material.
The information contained herein is intended for use by informed individuals who can and must determine its fitness for their purpose.